Skip to main content
. 2015 Sep 2;290(42):25636–25645. doi: 10.1074/jbc.M115.671925

FIGURE 3.

FIGURE 3.

Effect of low temperature incubation on corrected CFTR-mediated Cl− secretion in CFBE F508del-CFTR cells after treatment with C4, C18, or the combination C4 + C18. Original short-circuit current recordings (A) in untreated cells or after incubation of C4, C18, and C4 + C18 (10 μm, 16 h) in cells kept at 37 °C, or at 27 °C (B) as indicated. C, corresponding ΔIsc response. Data are expressed as the CFTRinh172 sensitive short-circuit current (ΔIsc) calculated by subtracting the Isc after CFTRinh172 treatment from the peak forskolin/genistein-stimulated Isc. Dots are associated with a 27 °C incubation. Statistical significance is presented as follows: ns, no significant difference; *, p < 0.05; **, p < 0.01; ***, p < 0.001 (n = 5–9 for each condition) compared with control condition (n = 8 for 37 °C and 5 for 27 °C). Amiloride (100 μm) was kept during the duration of the whole experiment to avoid interference of ENaC-mediated Na+ currents.