FIGURE 7.
Effect of double knockdown Hsp27 and Hsp40 on CFTR-mediated Cl− secretion in CFBE F508del-CFTR cells. A, original short-circuit current recording in untreated cells or after incubation with siRNA Hsp27, siRNA Hsp40, or the combination siRNA Hsp27 + siRNA Hsp 40 (72 h, 37 °C). B, corresponding ΔIsc response. Data were expressed as the CFTRinh172 sensitive short-circuit current (ΔIsc) calculated by subtracting the Isc after CFTRinh172 treatment from the peak forskolin/genistein-stimulated Isc. Statistical significance was presented as follows: ns, no significant difference; *, p < 0.05; **, p < 0.01; ***, p < 0.001 (n = 4–8 for each condition) compared with control condition (n = 4). Amiloride (100 μm) was kept during the duration of the whole experiment to avoid interference of ENaC-mediated Na+ currents.