FIGURE 3.

Binding of TM peptides to immobilized Rho. Examples of the binding response curves are shown for TM4, TM5, and scrambled TM4. Rho was immobilized onto an EnSpire-LFB 384-well plate, and binding responses of TM peptides delivered to the plate wells in concentrations ranging from 0.006 to 2 μm were measured with an EnSpire plate reader integrated with a Corning label-free module. After reactions reached equilibria, the responses from 10 measurements were averaged for each data point. Averaged data from three independent experiments were plotted as y values with corresponding concentrations of tested peptide as x values. Binding curves were fitted by the Hill function with Origin 8.1 software.