FIGURE 9.
Effects of TM peptides on Rho function. A, rates of in vitro Gt activation with Rho purified by gel filtration after treatment with TM peptides. B, effects of selected TM peptides on light-stimulated cAMP accumulation in cells expressing opsin. HEK-293 (mOpsin·Rluc and mOpsin·Venus) cells were regenerated with 9-cis-retinal overnight (control cells were not incubated with 9-cis-retinal) and treated with 1 μm TM peptides for 1 h at 37 °C following stimulation with forskolin and light illumination. Control cells, not exposed to 9-cis-retinal, underwent the same protocol. cAMP levels were detected as described under “Materials and Methods.” Each experiment was performed in triplicate.