TABLE 2.

Binding of TM peptides to immobilized Rho

Freshly prepared TM peptides in concentrations ranging from 0.006 to 2 μm were added to an EnSpire-LFB 384-well plate with Rho immobilized at the bottom of each well. Binding responses were measured over a time course by an EnSpire plate reader integrated with a Corning label-free module. Binding responses were stabilized for about 30 min after peptides were added, and 10 data points were averaged to obtain the binding response for each well. Averages and standard deviations of the binding responses calculated from three repeats of each peptide concentration were plotted as y values and error bars in the binding chart, with the concentrations of peptide tested as the x values. Binding curves were fitted by the Hill function from Origin 8.1 software. NA, not associated.

TM peptides	Kd
	μm
TM1	0.78 ± 0.44
TM2	1.18 ± 0.53
TM3	0.64 ± 0.13
TM4	0.47 ± 0.27
TM5	0.62 ± 0.03
TM6	NA
TM7	NA
TM4 scrambled	NA