FIGURE 5.

Comparison of the CofB structure with the major pilin CofA and the minor pseudopilin GspK. A, topology diagrams of CofB (pilin domain), CofA, and GspK. Pilin domains are shown in blue with the αβ-loop in pink. The β-repeat and β-sandwich domains of CofB are shown as boxes. The GspK α-domain is shown in green. B, schematic representations of CofB, CofA (Protein Data Bank entry 3S0T) and GspK (3CI0) structures. CofB is rotated 90° relative to its orientation in Fig. 4, such that the C-terminal domains point away from the reader and are not visible. Disulfide bonds are shown in ball-and-stick representations with sulfurs colored yellow. C, surface representations of CofB, CofA, and GspK, colored as in A. Cysteine sulfurs are colored yellow, nitrogens are blue, and oxygens are red.