FIGURE 6.

PFKFB3 overexpression results in increased lactate production and Akt activity in HEK293 cells. HEK293 cells were transfected with PFKFB3 or vector control (Vec) 48 h prior to serum starvation for 2 h, followed by stimulation with or without 100 nm insulin for 20 min. A, prior to starvation, cell medium that was incubated on the cells for 16 h was collected and lactate levels were measured and normalized to vector control between experiments (n = 14). B, cell lysates were immunoblotted with the indicated antibodies and representative immunoblots are shown. C, quantification of insulin-stimulated Akt and AS160 phosphorylation (data in B) is shown (phospho/total), normalized to insulin-stimulated cells transfected with vector control (n = 11–21). *, p < 0.05; **, p < 0.01; ****, p < 0.0001 versus Vec control, error bars represent S.E.