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. 2015 Aug 31;290(43):25891–25906. doi: 10.1074/jbc.M115.684704

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Kirrel2 is cleaved and shed from MIN6 cells. A, lysates of MIN6 cells transfected with Kirrel2-V5 (+) or with control (−) plasmids were resolved with reducing (+DTT) or non-reducing (−DTT) SDS-PAGE and immunoblotted (IB) with anti-V5 antibody. B, total lysates and cytoplasmic and membrane-enriched fragments of MIN6 cells transfected with control (−) or Kirrel2-V5 (+) vectors were immunoblotted with anti-V5 tag, E-cadherin, and GAPDH antibodies. C, lysates and concentrated supernatants of MIN6 cells transfected with control (−) or Kirrel2-V5 (+) vectors were immunoblotted with antibodies against the extracellular domain of Kirrel2. D, MIN6 cells, transfected with control (−) or Kirrel2-V5 (+) vectors, were treated with DMSO or γ-secretase inhibitor DAPT (10 μm) for 16 h. Lysates were immunoblotted with anti-V5 antibodies. E, lysates and concentrated supernatants of MIN6 cells, expressing wild type or mutant Kirrel2-V5, were immunoblotted with anti-Kirrel2 antibody.