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. 2015 Aug 27;290(43):26007–26020. doi: 10.1074/jbc.M115.669804

FIGURE 5.

FIGURE 5.

DEER analysis for trimeric LHCII versions with spin labels near the N terminus. Insets in A, C, and E show modulation depth-scaled zooms into the first 1 μs to highlight changes. A, primary experimental (DEER) data for trimeric LHCII versions 3/34 (black) and 3/59 (red) in n-octyl-β-d-glucoside (OG) micelles. B, inter-spin distance distributions between labels at residues 3/34 (black) and 3/59 (red). C, primary experimental (DEER) data for LHCII versions 7/34 (black) and 7/59 (red). D, distance distributions for LHCII versions 7/34 (black) and 7/59 (red). The dashed red line is the fit by a bimodal Gaussian distribution used for deriving the distance restraint for 7/59. The width of the long-distance aggregate peak is an unstable parameter at the restricted length of the time domain data. E, primary experimental (DEER) data for LHCII versions 11/34 (black) and 11/59 (red). F, distance distributions for LHCII versions 11/34 (black) and 11/59 (red). The dashed black line is the fit by a bimodal Gaussian distribution used for deriving the distance restraint for 11/34.