FIGURE 3.

TREM2-Ig precipitates ApoE from serum. A, immunoprecipitation (IP) of cynomolgus macaque serum was performed in an experiment analogous to that shown in Fig. 1. The precipitated proteins are shown next to input (serum) following reducing SDS-PAGE and silver staining. ApoE was not visible on the silver-stained gel but ApoA-I was visible beneath the IgG light chain in both serum and the TREM2-Ig IP lane. B, Western blot confirmed the presence of ApoE and ApoA-I in the TREM2-Ig precipitate. The white arrowhead indicates the hIgG1 light chain band. IB, immunoblot.