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. 2015 Sep 15;290(43):26043–26050. doi: 10.1074/jbc.M115.679043

FIGURE 2.

FIGURE 2.

ApoA1 and apoB also bind to TREM2. A, a representative dot blot of TREM2 binding to apoA1 and apoB. Membrane strips spotted with 200 ng each of normal mouse IgG (mIgG), apoA1, or apoB were incubated with 80 nm hIgG-Fc, TREM1-Fc, or TREM2-Fc. Bound Fc protein was detected with biotinylated anti-Fc antibody. B, a representative dot blot showing binding specificity of TREM2 to apoE and not to two other LRP1 ligands, tPA or RAP. Membrane strips spotted with 200 ng each of mIgG, apoE3, tPA, or RAP were incubated with 80 nm hIgG-Fc, TREM1-Fc, or TREM2-Fc, and bound Fc protein was detected with biotinylated anti-Fc antibody. C, apoA1 competes with apoE for binding to TREM2. Membrane strips spotted with hIgG-Fc, TREM1-Fc, or TREM2-Fc were incubated with 10 nm apoE3 along with increasing concentrations of apoA1, and bound apoE was detected and quantified using biotinylated anti-apoE antibody. Data are plotted as mean ± S.E. (n = 3).