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. 2015 Sep 10;290(43):26059–26071. doi: 10.1074/jbc.M115.649509

FIGURE 4.

FIGURE 4.

A, steady state surface expression of murine IL-6R on major circulating immune cell subsets was analyzed by flow cytometry. Note that IL-6R expression on inflammatory monocytes (CD11b+, Ly6Chigh) is considerably higher than on neutrophils (CD11b+, Ly6G+), T and B cells (CD3+ and B220+, respectively). Leukocytes were pre-gated on CD45 prior to gating on different leukocyte subsets. One representative histogram is shown (n = 3 C57BL/6 mice). B, serum from ADAM10Δmyeloid mice (n = 17) was prepared from whole blood and murine sIL-6R was quantified by ELISA. ADAM10flox/flox littermates (n = 21) served as controls. C and D, serum sIL-6R levels in ADAM8−/− (n = 5, control n = 6) and DPPI−/− (n = 4, control n = 4) mice were measured as in B. As DPPI−/− animals were on an ApoE−/− background, ApoE−/− mice served as control. E, circulating sIL-6R in PLS patients and age-matched healthy individuals was quantified by ELISA (n = 9). Error bars represent S.D. n.s. = not significant.