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. 2015 Sep 8;290(43):26103–26113. doi: 10.1074/jbc.M115.679084

FIGURE 6.

FIGURE 6.

Analysis of receptors associated with Sdc1 and Sdc4 in HaCat keratinocytes and MCF10A breast epithelial cells. A, receptors shown previously (2) to comprise the functional complexes responsible for matrix chemokinesis (HER2, Sdc1, α3β1 integrin, α6β4 integrin) or EGF-chemokinesis (EGFR, Sdc4, α3β1 integrin, α6β4 integrin) of HaCat and MCAF10A cells are imunoprecipitated, then probed for the presence of either HER2 or EGFR. (*, Note splicing of blot for the purpose of re-arranging the sample order for MCF10A cells). B, α6β4 integrin is immunoprecipitated from MCF10A cell lysates in the presence or absence of SSTN210–240 or SSTN87–131, then probed for Sdc1, Sdc4, α3β1 integrin, HER2, EGFR, and α6β4 integrin. Each of the receptors is also immunoprecipitated using receptor-specific antibodies and stained to provide a receptor marker. C, quantification of α3β1 integrin co-precipitation with α6β4 integrin in the presence or absence of SSTN peptides (*, p < 0.01).