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Expression of the mucin and galectin family in HCT116 cells. A and B, mRNA levels of the mucin (A) and galectin (B) family in HCT116/Mock and HCT116/MUC1 cells were determined by DNA microarray analysis. C, lysates of HCT116/Mock and HCT116/MUC1 cells were subjected to SDS-PAGE, followed by Western blotting and detection with anti-MUC1-ND, anti-MUC1-CD, anti-galectin-1, anti-galectin-3, and anti-β-actin antibodies. β-Actin was used as a loading control.
