TABLE 2.
Quantified change in Ca2+ release properties for RyR2 channels expressed in HEK293 cells transfected with CaM
Numbers in parentheses indicate the percentages of statistically significant change in the measured properties for RyR2 Ca2+ release relative to RyR2 expressing cells transfected with CaM WT (p < 0.01, one-way analysis of variance). Transfections of RyR2 ΔCaMBD expressing cells with CaM variants did not change the Ca2+ release properties (Fig. 4, A–E). For comparison to RyR2 WT expressing cells, an average for all RyR2 ΔCaMBD experiments was calculated (Average RyR2 ΔCaMBD).
| CaM variant | Activation | Termination | Fractional release |
|---|---|---|---|
| % | % | % | |
| WT | 91 | 56 | 35 |
| N53I | 86 (−5) | 44 (−21) | 42 (20) |
| N97S | 87 (−4) | 39 (−30) | 48 (38) |
| D95V | 85 (−6) | 38 (−33) | 48 (38) |
| D129G | 87 (−4) | 40 (−28) | 47 (34) |
| (Average RyR2 ΔCaMBD) | 92 | 34 (−38) | 58 (65) |