Skip to main content
. 2015 Sep 8;290(43):26194–26203. doi: 10.1074/jbc.M115.678979

FIGURE 1.

FIGURE 1.

CRH and Ucn 3 increase the expression level of VEGF-A in the intestinal epithelium through CRHR1 and CRHR2, respectively. A, endogenous expression levels of CRHR1 and CRHR2 in NCM460 cells were determined by qPCR. The complementary DNA from human smooth muscle cells was used as a calibrator. B, expression levels of CRHR1 and CRHR2 were shown by immunofluorescence staining. C–F, ELISA was performed to measure VEGF-A levels in NCM460 cells (C and D; #, p < 0.05 versus CRH, $, p < 0.05 versus Ucn 3), mIECs (E; #, p < 0.05 versus CRH in CRHR1+/+ cells, $, p < 0.05 versus Ucn 3 in CRHR2+/+ cells), hCSMC and hASMC (F).