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. 2015 Sep 8;290(43):26194–26203. doi: 10.1074/jbc.M115.678979

FIGURE 2.

FIGURE 2.

Overexpression of CRHR1 or CRHR2 further enhances VEGF-A production. A, expression levels of CRH and Ucn 1–3 were determined by qPCR. The complementary DNA from human smooth muscle cells was used as a calibrator. B, expression levels of CRHR1 and CRHR2 in NCM460 cells overexpressing CRHR1 or CRHR2 was determined by qPCR. The complementary DNA from NCM460 cells overexpressing empty vector (EV) was used as a calibrator. C, VEGF-A mRNA expression level was determined by qPCR. D and E, ELISA was performed to VEGF-A levels in NCM460 cells overexpressing CRHR1 or CRHR2. Data are mean ± S.D. *, p < 0.05 versus vehicle.