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. 2015 Sep 2;290(43):26235–26248. doi: 10.1074/jbc.M115.672097

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Ethanol extraction and solvent partition of 2 kg of fenugreek seed. The extraction started with extracting 2 kg of dried seed powder of fenugreek with 20 liters of 95% ethanol for 20 h. The 519 g of concentrated and dried ethanol extract (FE-E) were further subjected to solvent partition with n-hexane, ethyl acetate, n-butanol, and water to give 262 g of n-hexane fraction (FE-E-Hx), 28.7 g of ethyl acetate fraction (FE-E-EA), 46 g of n-butanol fraction (FE-E-But), and 174 g of water fraction.