FIGURE 11.

N55 does not affect the binding of GLP-1(7–36)-amide to GLP-1R. Total (black circles) and nonspecific (black squares) binding of ¹²⁵I-labeled GLP-1(7–36) to GLP-1R-V2R membrane in the absence (A) or presence (B) of 7.7 μm N55., specific binding of ¹²⁵I-labeled GLP-1(7–36) to GLP-1R-V2R membrane in the absence (black circles) or presence (black squares) of 7.7 μm N55. Specific bindings are obtained by subtracting nonspecific bindings from total bindings. All data are mean ± S.E. of duplicates from three independent experiments.