FIGURE 13.

Trypsin cleavage activity is not affected by N55. To examine whether the presence of N55 affects trypsin cleavage activity, we used trypsin activity colorimetric test (BioVision). Briefly, trypsin activity was assayed with and without 77 μm N55; 2 μl of trypsin substrate was added to a well in a 96-well plate containing 48 μl of 0.000125% trypsin in the presence (blue squares) or absence (open circles) of 77 μm of N55. Reactions were conducted at room temperature, and the extent of cleavage was monitored by real time reading at A₄₀₅.