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. 2015 Sep 2;290(43):26235–26248. doi: 10.1074/jbc.M115.672097

FIGURE 2.

FIGURE 2.

Effect of extracts from fenugreek seeds on GLP-1 receptor endocytosis to the titration of GLP-1(7–36)-amide. A, fluorescent images of effect of vehicle, 0.1 mg/ml ethanol extract of fenugreek seed (FE-E), 1 nm GLP-1(7–36)-amide, and 1 nm of GLP-1(7–36)-amide plus 0.1 mg/ml FE-E on the distribution of β-arrestin2-GFP in U2OS cells co-expressing GLP-1R-V2R. B, dose-response curve of receptor endocytosis in response to increasing concentrations of GLP-1(7–36)-amide in the presence (black triangles) or absence (black circles) of 0.1 mg/ml FE-E. GLP-1R endocytosis elicited by 1 nm GLP-1(7–36)-amide and in the presence of increasing concentration of FE-E (C), n-hexane partition fraction of FE-E (FE-E-Hx) (D), ethyl acetate partition fraction of FE-E (FE-E-EA) (E) and n-butanol partition fraction of FE-E (FE-E-Bu) (F). Data are mean ± S.E. from triplicate of three independent experiments.