FIGURE 3.

Extracts from fenugreek seed-enhanced GLP-1(7–36)-amide stimulated cAMP production in RINm5F cells. cAMP production response to GLP-1(7–36)-amide titration in RINm5F cells expressing RG-cAMP sensor fusion protein in the presence of 0.1 mg/ml hexane partition fraction (FE-E-Hx) (green squares) or ethyl acetate fraction (FE-E-EA) (red triangles) or vehicle (black circles). Activity was expressed as cAMP production % of the maximal response and was calculated as 100·(A − B)/(A − M), where A = BRET ratio by 10 pm GLP-1(7–36)-amide; B = BRET ratio by indicated concentration of GLP-1(7–36)-amide in the presence of indicated concentration of extract, and M = BRET ratio by 0.25 μm of GLP-1(7–36)-amide. Data are mean ± S.E. of triplicates of three independent experiments.