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. 2015 Sep 2;290(43):26235–26248. doi: 10.1074/jbc.M115.672097

FIGURE 5.

FIGURE 5.

Reverse phase chromatography profile of FE-EA-F2 versus activity to enhance cAMP production by GLP-1 in RINm5F cells stably expressing RG-cAMP sensor. 3 g of FE-EA-F2 were fractionated by preparative RP-HPLC (LiChrospher® select B column 250 × 25 mm, 10 μm) and resolved into 72 fractions. Each fraction (0.01 mg/ml) was tested for its ability to enhance cAMP production by 1 nm GLP-1(7–36)-amide in RINm5F cells stably expressing RG-cAMP. Activity was expressed as increased cAMP production (% of the maximal response) and was calculated as 100×(A − B)/(C − M), where A = BRET ratio by 1 nm GLP-1(7–36)-amide; B = BRET ratio by 1 nm GLP-1(7–36)-amide in the presence of 0.01 mg/ml indicated fraction; C = BRET ratio by 10 pm GLP-1(7–36)-amide; and M = BRET ratio by 0.25 μm GLP-1(7–36)-amide. Black diamonds represent activity and red triangles indicate weight (mg) for each fraction.