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Determination of kinetic parameters for CalS8 and CalS8 mutant P294S mutant using standard conditions with saturating (20 mm) NAD+ and varied (from 0.02 to 10 mm) UDP/TDP-Glc concentrations at 30 °C
| Enzyme/Substrate | kcat | Km | kcat/Km × 10−3 |
|---|---|---|---|
| min−1 | μm | ||
| CalS8/UDP-Glc | 1.6 ± 0.1 | 930.0 ± 128.0 | 1.7 |
| CalS8/TDP-Glc | 1.3 ± 0.1 | 44.9 ± 7.4 | 29.0 |
| P294S/TDP-Glc | 1.0 ± 0.1 | 31.9 ± 3.3 | 31.4 |
