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. 2015 Nov 16;10(11):e0142835. doi: 10.1371/journal.pone.0142835

Fig 4. Coagulation time and inhibition assay.

Fig 4

(A) Coagulation times of RBCs, platelets, PMNs, MNCs, and MPs from healthy subjects (n = 20), Non-D (n = 25), CAPD (n = 18) and HD patients (n = 23) were evaluated. Blood cells from uremic patient (especially HD) had more procoagulant activity than that from healthy subjects. (B) ECs cultured for 24 h in uremic serum or mixed toxins showed more procoagulant activity than those in normal serum. Data are representative of triplicate independent experiments. PCA of blood cell and MPs in 23 HD patients (C) and cultured ECs in uremic serum for 24h (D) was detected in the absence or presence of 128 nM lactadherin. Lactadherin almost entirely inhibited PCA supported by blood cell, EC and MP. Data are displayed as mean ± SD (*P < 0.001, #P < 0.001). MPs: microparticles; PMN: polymorphomuclear cell; MNC: mononuclear cell; PCA: procoagulant activity; Non-D: Non-dialysis; CAPD: continuous ambulatory peritoneal dialysis; HD: haemodialysis.