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. 2015 Nov 16;10(11):e0142835. doi: 10.1371/journal.pone.0142835

Fig 5. Formation and inhibition assays of procoagulant enzyme complexes.

Fig 5

FXa and thrombin production of 2×105 RBCs (A), 2×104 platelets (B), 2×104 PMN (C), 2×104 MNC (D), 2×104 ECs (E) and 10 μl-MPs from each group (20 healthy subjects, 25 Non-D, 18 CAPD and 23 HD) are shown. Intrinsic FXa formation was measured in the presence of FIXa, FVIII and thrombin. Extrinsic FXa production was assessed in the presence of FVIIa. Thrombin generation was investigated in the presence of FXa and FVa. Results displayed are mean ± SD (*P < 0.001, #P < 0.001). (F) The capacity of 128 nM lactadherin to block procoagulant enzyme complexes on cells/MPs from 23 HD patients, and ECs incubated in uremic plasma was evaluated. In each kind of cell, lactadherin decreased activity of the procoagulant enzyme complexes by approximately 80%. Non-D: Non-dialysis; CAPD: continuous ambulatory peritoneal dialysis; HD: haemodialysis.