FIGURE 5.

Fitness metric and relative dissociation constant error. a, relative dissociation constant as a function of fitness metrics. The vertical dashed line represents the average fitness metric for stop codon positions. b, standard error as a function of fitness metric for different numbers of unselected counts (red, 10; blue, 20; green, 30; orange, 50; purple, 100; black, 500). Fitness metrics associated with lower number of counts have higher error. c, relative dissociation constant error as a function of relative dissociation constant for different numbers of unselected counts. As the relative dissociation constant increases the amount of error increases.