FIGURE 6.

TROP2 expression in MDA-MB-231 cells enhances migration and invasion. a and b, MDA-MB-231 cells were transfected with scramble siRNA or siRNA targeting TROP2 for 24 h. The mRNA (a) and protein levels (b) of TROP2 were measured by real-time PCR and Western blotting. n = 3. **, p < 0.01; ***, p < 0.001 versus control. c, suppression of MDA-MB-231 migration by silencing TROP2. MDA-MB-231 cells were transfected with scramble siRNA or siRNA targeting TROP2 and then subjected to a transwell migration assay. The cells were allowed to migrate toward serum for 8 h. Triplicate wells were used for each condition in three independent experiments. ***, p < 0.001 versus control. d, silencing TROP2 reduced the migration of highly invasive breast cancer cells. MDA-MB-231 cells were transfected with scramble siRNA or TROP2 siRNA and subjected to a wound-healing assay. Representative photographs at the indicated time points from three independent experiments, with each performed in triplicate wells. Magnification, 10×. e, suppression of MDA-MB-231 invasion by silencing TROP2. MDA-MB-231 cells were transfected with scramble siRNA or siRNA targeting TROP2 and then seeded in a Matrigel-coated Boyden chamber and subjected to a transwell invasion assay. The cells were allowed to migrate toward serum for 20 h. Triplicate wells were used for each condition in three independent experiments. ***, p < 0.001 versus control.