FIGURE 8.

a, 10⁵ MDA-MB-231 cells were labeled with 50 nm biotinylated m7e6 IgG (orange), 50 nm biotinylated m7e6 Fab (cyan), or nude (blue) in buffer PBS plus 1 g/L bovine serum albumin for 30 min at room temperature. After washing cells were secondarily labeled with streptavidin-phycoerythrin and processed by flow cytometry. b, bar graphs indicating the average (n = 3) Alamar Blue assay absorbance (570 nm) in cells treated with PBS (control) or increasing concentration of m7E6 IgG (10–40 μg/ml). c, bar graphs indicating the average (n = 3) lactate dehydrogenase absorbance (590 nm) in control and m7EG IgG-treated cells. p values indicate the significance levels of the influence of increasing concentrations of the IgG treatment on proliferation or cytotoxicity levels determined using one-way analysis of variance.