TABLE 1.
Deamination t½ and yields for CPD formation in the FOS promoter in vitro and in vivo
CPD 1 | CPD 2 | CPD 3 | CPD 4 | |
---|---|---|---|---|
In vitro | ||||
t½ (h) | 4.8 ± 2.2 | 6.7 ± 3.2 | 3.2 ± 0.9 | 4.0 ± 1.0 |
Relative rates | 1 | 0.72 ± 0.47 | 1.5 ± 0.8 | 1.2 ± 0.6 |
Relative yield (curve fit) | 1 | 0.31 ± 0.06 | 0.38 ± 0.07 | 0.36 ± 0.06 |
Relative yield (gel, 48 h) | 1 | 0.39 | 0.47 | 0.34 |
In vivo 1 | ||||
t½ | 7.8 ± 4.6 | 4.7 ± 1.3 | 3.9 ± 0.9 | |
Relative rates | 1 | 1.7 ± 1.1 | 2.0 ± 1.3 | |
Relative yield (curve fit) | 1 | 0.62 ± 0.15 | 0.63 ± 0.15 | |
Relative yield (gel 70 h) | 1 | 0.15 | 0.48 | 0.70 |
In vivo 2 | ||||
t½ | 15.8 ± 6.8 | 5.3 ± 1.5 | 7.6 ± 4.2 | |
Relative rates | 1 | 3.0 ± 1.5 | 2.1 ± 1.5 | |
Relative yield (curve fit) | 1 | 0.40 ± 0.11 | 0.32 ± 0.10 | |
Relative yield (gel 70 h) | 1 | 0.14 | 0.47 | 0.67 |
Average of 1 and 2 | ||||
t½ | 11.8 ± 8.2 | 5.0 ± 2 | 5.7 ± 4.3 | |
Relative rates | 1 | 2.1 ± 1,0 | 2.0 ± 1.0 | |
Relative yield (curve fit) | 1 | 0.48 ± 0.09 | 0.43 ± 0.09 | |
Relative yield (gel 70 h) | 1 | 0.14 | 0.47 | 0.67 |
Rate change | ||||
In vivo/in vitro | 0.46 ± 0.2 | 0.63 ± 0.30 | 0.78 ± 0.23 | |
Relative to CPD1 | 1 | 1.4 ± 0.9 | 1.70 ± 0.9 | |
Predicted | ||||
Position 2: −1 | 1 | 4.3 ± 0.7 | 4.3 ± 0.7 | |
Position 3: 0 | 1 | 2.5 ± 0.3 | 2.5 ± 0.3 | |
Position 4: 1 | 1 | 0.5 ± 0.1 | 0.5 ± 0.1 |