FIGURE 6.
MLIP is required for proper cardiac response to stress. A, heart to body weight ratio of 12-week-old Mlip+/+ and Mlip−/− female mice treated with isoproterenol (40 mg·kg−1·day−1) for 9 days (n = 6 per group, means ± S.D.). **, p < 0.01; *, p < 0.05 versus genotype-matched saline control. Student's t test was used. B, heart to body weight ratio of 12-week-old Mlip+/+ and Mlip−/− male mice treated with isoproterenol (40 mg·kg−1·day−1) for 9 or 15 days (n = 3–8 per group, means ± S.D.). *, p < 0.05 versus genotype-matched saline control. Student's t test was used. C, hematein/eosin staining of Mlip+/+ and Mlip−/− male hearts after 15 days of isoproterenol infusion. Scale bars, 2.5 mm. D, Masson's Trichrome staining of Mlip+/+ and Mlip−/− male hearts after 15 days of isoproterenol infusion. Scale bars, 200 μm. E, cardiac section of Mlip+/+ and Mlip−/− male hearts after 15 days of isoproterenol infusion, stained with WGA. Scale bars, 50 μm. Measure of cardiomyocyte cross-section area (CSA) based on WGA staining (n = 3–4 per group, means ± S.D.). **, p < 0.01; *, p < 0.05. Student's t test was used. F, representative Western blot and quantification of total and phosphorylated Akt and mTOR in Mlip+/+ and Mlip−/− female hearts treated with isoproterenol for 9 days (n = 3–4 per group, means ± S.D.). **, p < 0.01; *, p < 0.05. Student's t test was used.