FIGURE 4.

The CDC25 and RA2 domains are required for IL-6 mRNA expression. A, IL-6 mRNA levels in primary WT and PLCϵ KO astrocytes treated with thrombin (5 nm) or vehicle (control) for 1 h were assessed by q-PCR. Fold increase is expressed relative to the WT or KO averaged controls. Data shown are the mean ± S.E. of values (n = 6) from three independent experiments. B, IL-6 mRNA levels were measured by q-PCR in PLCϵ KO astrocytes infected with WT PLCϵ, CDC25Δ, or RA2 mutant (K2150E) adenovirus followed by thrombin (5 nm) treatment for 1 h. Fold increase is expressed relative to its own control. Data shown are the mean ± S.E. of values (n = 6) from three independent experiments. *, p < .05 and **, p < .01 between control and thrombin treatment; #, p < .05 between thrombin treatments, one-way ANOVA.