FIGURE 6.

PKD is activated at the Golgi in a PLCϵ-dependent manner. Following transfection of Golgi-DKAR (1.5 μg) in WT astrocytes (A) or in KO astrocytes (B), the FRET response (CFP/FRET) was measured over time after the addition of thrombin (5 nm). Data quantitated as the mean ± S.E. (n = 8) of four independent experiments. C, plasma membrane-DKAR (1.5 μg) was transfected into WT astrocytes. The FRET response (CFP/FRET) was measured over time after the addition of thrombin (5 nm). Data are quantitated as the mean ± S.E. (n = 8) of four independent experiments.