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IRI activity of Con A in HEPEs buffer. (A) Effect of (5 mg mL–1) CaCl2 addition. [Con A] = 5 mg mL–1, [CaCl2] = 5 mg mL–1, [PEG] = 5 mg mL–1. (B) Concentration dependence of Con A with 5 mg mL–1 CaCl2. Error bars represent ± SD from a minimum of three repeats. MLGS = mean largest grain size relative to HEPES buffer or HEPES and calcium chloride buffer control. **, p < 0.01 relative to PEG control.
