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Metal ion activation of Nisin A IRI activity upon addition of nickel and zinc acetate at 5 mg mL–1. (A) PBS buffer, pH 7.4. (B) Acetate buffer, pH 5. Error bars represent ± SD from a minimum of three repeats. MLGS = mean largest grain size relative to PBS buffer, pH 5, acetate buffer controls, or buffer with nickel acetate added only. **, p < 0.01 relative to Nisin in PBS buffer only.
