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. 2015 Nov 16;10(11):e0143114. doi: 10.1371/journal.pone.0143114

Fig 1. Ovarian tissue culture under 4 different culture conditions.

Fig 1

A mouse ovary at 14 dpp was cut into 8 pieces and subjected to organ culture. Ovarian tissue pieces were directly placed onto a floating membrane filter on IVG medium and cultured with or without activin A (Membrane culture). For our 3-D culture system, Matrigel diluted with IVG medium (1:1) was placed on a membrane filter to make a Matrigel drop. A single ovarian tissue was placed on top of each drop and cultured with or without activin A treatment. The tissues were cultured for 10 days under four different culture conditions: Control (C: Membrane/activin-), with activin A (A: Membrane/activin+), with Matrigel but no activin A (M: Matrigel/activin-), and with Matrigel and activin A (M+A: Matrigel/activin+).