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. 2015 Nov 16;10(11):e0143114. doi: 10.1371/journal.pone.0143114

Fig 5. Chromatin configuration of GV oocytes isolated from follicles in ovarian tissues cultured under 4 different conditions.

Fig 5

(a) Chromatin configurations of mouse GV oocytes after Hoechst staining (400x). By the presence or absence of a ring of condensed chromatin around the nucleorus, the oocytes were categorized into three groups: NSN (non-surrounded nucleolus), SN (surrounded nucleolus) and Int (intermediate stage). The SN configuration represents a stage of GV oocyte that is more advanced toward ovulation. Each square represents a magnified chromatin configuration. (b) Distribution of chromatin configuration patterns in the oocytes isolated from the follicles on Days 0, 6 and 10 of culture. On Day 6, the GV chromatin condensation was more advanced under the 3-D Culture (M, M+A) compared to under the Membrane Culture (C, A) (a-c: P<0.05; b,c: P<0.02). By Day 10 of culture, almost all of full-size GV oocytes progressed to the SN condfiguration regardless of the 4 different culture conditions.