Skip to main content
. 2015 Nov 16;10(11):e0143114. doi: 10.1371/journal.pone.0143114

Fig 6. Developmental competence of full-size GV oocytes grown under 3-D culture system.

Fig 6

(a) After 10 days of the 3-D ovarian tissue culture (M+A), GV oocytes surrounded by granulosa cells were isolated from the antral follicles for in vitro maturation (IVM). (b) To observe germinal vesicles (arrows) in GV oocytes, surrounding granulosa cells were mechanically removed. (c) After IVM, granulosa cell layers surrounding the oocytes underwent expansion. (d) To observe first polar bodies (arrows) in matured MII oocytes, expanded granulosa cell layers were removed after IVM. (e) In vitro matured oocytes with expanded granulosa cells were subjected to in vitro fertilization (IVF). Six hrs after IVF, two pronuclei (arrows) were observed in each fertilized oocyte. (f) Twenty-four hrs after IVF, some of fertilized oocytes developed to the 2-cell stage embryos. (g) The 2-cell embryos were transferred to surrogate mothers to obtain full-term offspring with black coat color. Bar = 40 μm.