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Cdc37 does not bind to PKCδ-Hsp90 complex. HeLa cells were serum-starved for 24 h and then treated with (−)-maackiain (30 μm) or 17-AAG (5 μm) for 24 h before harvesting the cells. The extracts were immunoprecipitated (IP) with PKCδ antibody. Rabbit control IgG was used as a control. Cdc37 was detected by immunoblot analysis (IB). The arrow indicates Cdc37, and a band derived from IgG is indicated with an asterisk.
