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Effect of quercetin on the interaction of PKCδ with Hsp90. HeLa cells were serum-starved for 24 h and then treated with quercetin (50 μm) or 17-AAG (1 μm) for 1 h before harvesting the cells. The extracts were immunoprecipitated (IP) with PKCδ antibody. After centrifugation at 3,000 rpm for 2 min at 4 °C, Hsp90 was detected by immunoblot analysis (IB).
