TABLE 1.
Primer/probe name | Sequence |
---|---|
Human H1R mRNA | |
Sense primer | 5′-CAGAGGATCAGATGTTAGGTGATAGC-3′ |
Antisense primer | 5′-AGCGGAGCCTCTTCCAAGTAA-3′ |
Probe | FAMa-CTTCTCTCGAACGGACTCAGATACCACC-TAMRAb |
Rat H1R mRNA | |
Sense primer | 5′-TATGTGTCCGGGCTGCACT-3′ |
Antisense primer | 5′-CGCCATGATAAAACCCAACTG-3′ |
Probe | FAM-CCGAGAGCGGAAGGCAGCCA-TAMRA |
Rat IL-4 mRNA | |
Sense primer | 5′-CAGGGTGCTTCGCAAATTTTAC-3′ |
Antisense primer | 5′-CACCGAGAACCCCAGACTTG-3′ |
Probe | FAM-CCCACGTGATGTACCTCCGTGCTTG-TAMRA |
a FAM, 6-carboxyfluorescein.
b TAMRA, tetramethylrhodamine.