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. 2015 Sep 15;290(45):27370–27383. doi: 10.1074/jbc.M115.687293

FIGURE 1.

FIGURE 1.

The Bombyx E93 gene. A, the developmental changes in E93 mRNA levels in the fat body were determined by qPCR from day 2 of the fourth instar (4L-2) to day 2 of the prepupal stage (PP2). 4M, the 4th larval molting stage; W, the wandering stage. B, spatial distribution analysis of E93 expression at the wandering stage. MT, malpighian tubules; MG, midgut; FB, fatbody; EP, epidermis; G, gonad; B, brain; NC, nerve cord; WD, wing disc; PG, prothoracic gland; PSG, post silk gland. C, significant domains of Bobmyx E93: LLQHLL, PLSLSAK, HTH-1, and HTH-2. D, alignment of the predicted amino acid sequences of the HTH-1 and HTH-2 domains of E93 proteins from B. mori (Bm), Apis mellifera (Am; E93 was early cognized as Mblk1), Tribolium castaneum (TC), D. melanogaster (Dm), Culex quinquefasciatus (Cq), and Blattella germanica (Bg). Black and light gray shading indicate identical and similar amino acid residues, respectively.