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. 2015 Sep 15;290(45):27370–27383. doi: 10.1074/jbc.M115.687293

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — E93 RNAi decreases ecdysteroid titer and disrupts 20E signaling in the fat body. dsRNA (30 μg/larva) was injected into staged larvae at the initiation of the wandering stage. GFP dsRNA (30 μg/larva) was used as a control. All the experiments were performed 24 h after dsRNA treatment. A, a comparison of ecdysteroid titer. B–B′, qPCR analysis of 20E-response genes (B) and Western blot analysis of the protein levels of some 20E-response genes (B′) in the fat body. C and D, qPCR analysis of 20E-induced Atg genes (C) and apoptosis genes (D) in the fat body.