FIGURE 3.

SUPT16H interferes with association of P-TEFb with Tat-LTR. A, HEK293 cells stably expressing shSUPT16H (sh2) or shNT were transiently transfected with a pQCXIP-FLAG-Tat vector and subjected to IP assays using an anti-FLAG antibody. Endogenous CCNT1 protein level in cell lysate and precipitated samples were analyzed by Western blots using an anti-CCNT1 antibody. B, ChIP assays were performed against CCNT1 in shSUPT16H (sh2) or shNT expressing HEK293 cells that were infected with VSV-G pseudo-typed HIV-1 NL4–3-Luc (dEnv) viruses. The results throughout were from one representative from three independent experiments.