FIGURE 3.

Antagonist efficacy of JMV compounds toward ghrelin-promoted IP1 production, β-arrestin2 recruitment, and ERK1/2 phosphorylation. IP1 production (A), β-arrestin2 recruitment (B), and ERK1/2 phosphorylation (C) were measured as described in Figs. 1 and 2 and under the “Experimental Procedures.” HEK293T cells expressing the GHS-R1a were stimulated with ghrelin at 10⁻⁸ m in the absence or in the presence of 10⁻⁶ m JMV compounds. Data are expressed as the percentage of maximal ghrelin-induced stimulation. Bars and error bars represent the mean ± S.E. of three independent experiments, each performed in triplicate. Statistical significance between stimulated and non-stimulated cells was assessed using a one way ANOVA followed by Dunnett's post hoc test (***, p < 0.001; **, p < 0.01; *, p < 0.1).