FIGURE 5.

Efficacy and potency of ghrelin, MK-0677, and JMV 1843 toward G_q activation and IP1. A, dose-dependent G_q activation measured by BRET² in HEK293T cells co-expressing both the GHS-R1a and G_q biosensor. B, dose-dependent IP1 measured by HTRF in HEK293T cells expressing the GHS-R1a. The zero value corresponds to basal non-stimulated HEK293T cells expressing the GHS-R1a. Curves are representative of three independent experiments, each performed in triplicate. Values of EC₅₀ and E_max are reported in Table 2.