FIGURE 1.

A, schematic representation of reconstituted TP-DNA. φ29 TP-DNA is represented with a TP linked at each 5′ end. At the first step, the 3′-5′ exonuclease activity of Vent DNA polymerase degrades the 3′ ends of the TP-DNA. The resulting ssDNA region is further hybridized to 29-mer single-stranded oligonucleotides containing the φ29 right replication origin sequence and variants in the 3′ terminal thymines. B, 29-mer oligonucleotides containing wild-type and variants of the φ29 right replication origin sequence. The thymines changed into adenine are indicated in black letters.