Figure 2.
1,25(OH)2D3 regulation of MLCK activation and paracellular permeability is dependent on VDR. (A) Western blot analyses of HCT116 cells transfected with scramble or hVDR-specific siRNA followed by various treatments as indicated. The blots were incubated with a number of antibodies as shown. (B) Transepithelial resistance (TER) of HCT116 monolayers transfected with scramble or hVDR-specific siRNA under different treatments as indicated. *P<0.05; ** P<0.01 vs. corresponding Ctrl or 1,25-VD; # P<0.05 vs. corresponding scramble.