Table 2.
Bioactive Molecules in TCMs Form Particles and Inhibit Model Enzymes
| IC50 (μM) vs |
||||||
|---|---|---|---|---|---|---|
|
β-lactamase |
MDH |
|||||
| compound | no detergenta | with detergentb | no detergent | with detergent | CAC (μM)c | radius (nm) |
| curcumin | 12 | >30 | 9 | >30 | 17 ± 0.44 | 465 ± 30 |
| kaempferol | 47 | >100 | 61 | >100 | 81 ± 26 | 61 ± 20 |
| physcion | 7 | >20 | 11 | >20 | 0.32 ± 0.025 | 320 ± 83 |
| silibinin | NDd | ND | 12 | >100 | 0.47 ± 0.15 | 53 ± 16 |
| emodin | 27 | >100 | 28 | >100 | 28 ± 2 | 231 ± 61 |
| diphyllin | 55 | >100 | 49 | >200 | 40 ± 4.0 | 269 ± 49 |
| bufalin | 93 | >200 | 101 | >200 | 51 ± 3 | 1317 ± 394 |
| brazilin | 302 | >500 | 1.6 | 84 | 57 ± 7 | 52 ± 10 |
| puerarin | ND | ND | 135 | 156 | 135 ± 40 | 78 ± 25 |
| delphinidin | 72 | 148 | 27 | >100 | ND | ND |
| equol | 127 | >500 | ND | ND | ND | ND |
| canadine | ND | ND | ND | ND | 95 ± 11 | 52 ± 14 |
| phlorizin | ND | ND | ND | ND | ND | ND |
| noscapine | ND | ND | ND | ND | ND | ND |
a
50 mM potassium phosphate, pH 7.0.
b
50 mM potassium phosphate, pH 7.0 + 0.01% (v/v) Triton X-100.
c
Critical aggregation concentration determined by dynamic light scattering.
d
Not detected to precipitation limit.