Table 1. Plasmid concentrations and copy number determination.
| Virus target insertion | Vector | Concentration (ng/μL) | avg ± SD (ng/ μL) | Final (ng/ μL) | Weight per plasmid copy* (ng) | Copies/μL | |
|---|---|---|---|---|---|---|---|
| Qubit | ImageJ | ||||||
| Adeno | pCR2.1 | 8.0 | 7.8 | 7.9 ± 0.2 | 7.5 | 4.17 x 10−9 | 1.80 x 109 |
| InfA | pCR 2.1 | 10.1 | 11.5 | 10.8 ± 1.0 | 10 | 4.14 x 10−9 | 2.42 x 109 |
| InfA/H3N2 | pCR4 | 14.5 | 9.1 | 11.8 ± 3.8 | 10 | 4.37 x 10−9 | 2.29 x 109 |
| InfA/H1N1pdm09 | pCR2.1 | 11.1 | 11.8 | 11.5 ± 0.5 | 10 | 4.15 x 10−9 | 2.41 x 109 |
| InfB | pCR4 | 26.0 | 31.1 | 28.6 ± 3.6 | 30 | 4.17 x 10−9 | 7.19 x 109 |
| hPIV-1 | pCR4 | 13.2 | 8.7 | 9.5 ± 2.0 | 10 | 4.32 x 10−9 | 2.31 x 109 |
| hPIV-2 | pCR4 | 7.4 | 6.7 | 7.0 ± 0.5 | 7.5 | 4.15 x 10−9 | 1.81 x 109 |
| hPIV-3 | pCR4 | 38.8 | 35.0 | 36.9 ± 2.7 | 35 | 4.19 x 10−9 | 8.35 x 109 |
| RSV | pCR4 | 5.1 | 6.2 | 5.7 ± 0.8 | 5 | 4.17 x 10−9 | 1.20 x 109 |
| hRV | pCR4 | 32.7 | 29.8 | 31.3 ± 2.1 | 30 | 4.27 x 10−9 | 7.03 x 109 |
*Weight/copy was calculated using Geneious (v.8.1.3) which considers the exact sequence of the plasmid.